› 1xj5

X-ray diffraction
2.7Å resolution

X-RAY STRUCTURE OF SPERMIDINE SYNTHASE FROM ARABIDOPSIS THALIANA GENE AT1G23820

Released:
DOI: 10.2210/pdb1xj5/pdb
PDB entry 1xj5 coloured by chain, front view.
PDB entry 1xj5 coloured by chain, side view.
PDB entry 1xj5 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Entry authors: Wesenberg GE, Smith DW, Phillips Jr GN, Bitto E, Bingman CA, Allard STM, Center for Eukaryotic Structural Genomics (CESG)

Function and Biology Details

Reaction catalysed: 
S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195712 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spermidine synthase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 334 amino acids
Theoretical weight: 36.92 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZUB3 (Residues: 2-334; Coverage: 100%)
Gene names: At1g23820, F5O8.38, SPDSYN1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 88.814Å b: 95.207Å c: 89.164Å
α: 90° β: 104.96° γ: 90°
R-values:
R R work R free
0.188 0.188 0.245
Expression system: Escherichia coli

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