› 1xjv

X-ray diffraction
1.73Å resolution

Crystal structure of human POT1 bound to telomeric single-stranded DNA (TTAGGGTTAG)

Released:
DOI: 10.2210/pdb1xjv/pdb
PDB entry 1xjv coloured by chain, front view.
PDB entry 1xjv coloured by chain, side view.
PDB entry 1xjv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human POT1 bound to telomeric single-stranded DNA provides a model for chromosome end-protection.
Lei M, Podell ER, Cech TR
Nat Struct Mol Biol 11 1223-9 (2004)
PMID: 15558049

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114817 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Protection of telomeres protein 1 Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 33.2 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NUX5 (Residues: 6-299; Coverage: 46%)
Gene name: POT1
Sequence domains:
Structure domains: Nucleic acid-binding proteins
hT10 d(TTAGGGTTAG) Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.12 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2221
Unit cell:
a: 102.026Å b: 103.243Å c: 71.664Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.228 0.237
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

70 review citations

How shelterin protects mammalian telomeres.
Palm et al. (2008)
69 more

31 mentions without citation

Finding the end: recruitment of telomerase to telomeres.
Nandakumar et al. (2013)
30 more