› 1xkg

X-ray diffraction
1.61Å resolution

Crystal structure of the major house dust mite allergen Der p 1 in its pro form at 1.61 A resolution

Released:
DOI: 10.2210/pdb1xkg/pdb
PDB entry 1xkg coloured by chain, front view.
PDB entry 1xkg coloured by chain, side view.
PDB entry 1xkg coloured by chain, top view.
Source organism: Dermatophagoides pteronyssinus
Primary publication:
The crystal structure of recombinant proDer p 1, a major house dust mite proteolytic allergen.
Meno K, Thorsted PB, Ipsen H, Kristensen O, Larsen JN, Spangfort MD, Gajhede M, Lund K
J Immunol 175 3835-45 (2005)
PMID: 16148130

Function and Biology Details

Reaction catalysed: 
Broad endopeptidase specificity
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139978 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase 1 Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 35.42 KDa
Source organism: Dermatophagoides pteronyssinus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P08176 (Residues: 19-320; Coverage: 100%)
Gene name: DERP1
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases

Ligands and Environments

3 bound ligands:
Ligand YT3 1 x YT3
Ligand SO4 2 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 64.659Å b: 66.557Å c: 73.388Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.153 0.186
Expression system: Komagataella pastoris

Quick links

Citations

9 review citations

Nomenclature and structural biology of allergens.
Chapman et al. (2007)
8 more

9 mentions without citation

Relevant B cell epitopes in allergic disease.
Pomés A. (2010)
8 more