› 1xm3

X-ray diffraction
1.8Å resolution

Crystal structure of Northeast Structural Genomics Target SR156

Released:
DOI: 10.2210/pdb1xm3/pdb
PDB entry 1xm3 coloured by chain, front view.
PDB entry 1xm3 coloured by chain, side view.
PDB entry 1xm3 coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Kuzin A, Abashidze M, Vorobiev S, Forouhar F, Acton T, Ma L, Xiao R, Montelione G, Tong L, Hunt J, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128317 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiazole synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 264 amino acids
Theoretical weight: 28.45 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O31618 (Residues: 1-256; Coverage: 100%)
Gene names: BSU11690, thiG, yjbT
Sequence domains: Thiazole biosynthesis protein ThiG
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 144.536Å b: 120.722Å c: 74.244Å
α: 90° β: 108.32° γ: 90°
R-values:
R R work R free
0.212 0.212 0.237
Expression system: Escherichia coli BL21(DE3)

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