› 1xns

X-ray diffraction
2.8Å resolution

Peptide trapped Holliday junction intermediate in Cre-loxP recombination

Released:
DOI: 10.2210/pdb1xns/pdb
PDB entry 1xns coloured by chain, front view.
PDB entry 1xns coloured by chain, side view.
PDB entry 1xns coloured by chain, top view.
Source organism: Punavirus P1
Primary publication:
Peptide trapping of the Holliday junction intermediate in Cre-loxP site-specific recombination.
Ghosh K, Lau CK, Guo F, Segall AM, Van Duyne GD
J Biol Chem 280 8290-9 (2005)
PMID: 15591069

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114820 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Recombinase cre Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 36.58 KDa
Source organism: Escherichia virus P1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06956 (Residues: 20-343; Coverage: 95%)
Gene name: cre
Sequence domains: Phage integrase family
Structure domains:
loxP DNA Chain: C
Molecule details ›
Chain: C
Length: 35 nucleotides
Theoretical weight: 10.77 KDa
Source organism: Punavirus P1
Expression system: Not provided
loxP DNA Chain: D
Molecule details ›
Chain: D
Length: 34 nucleotides
Theoretical weight: 10.44 KDa
Source organism: Punavirus P1
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2221
Unit cell:
a: 106.078Å b: 121.529Å c: 177.799Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.196 0.265
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

The λ Integrase Site-specific Recombination Pathway.
Landy A. (2015)
3 more

1 mention without citation

Organometallic Pillarplexes That Bind DNA 4-Way Holliday Junctions and Forks.
Craig et al. (2023)