› 1xov

X-ray diffraction
1.8Å resolution

The crystal structure of the listeria monocytogenes bacteriophage PSA endolysin PlyPSA

Released:
DOI: 10.2210/pdb1xov/pdb
PDB entry 1xov coloured by chain, front view.
PDB entry 1xov coloured by chain, side view.
PDB entry 1xov coloured by chain, top view.
Source organism: Listeria phage PSA
Primary publication:
The crystal structure of the bacteriophage PSA endolysin reveals a unique fold responsible for specific recognition of Listeria cell walls.
Korndörfer IP, Danzer J, Schmelcher M, Zimmer M, Skerra A, Loessner MJ
J Mol Biol 364 678-89 (2006)
PMID: 17010991

Function and Biology Details

Reaction catalysed: 
Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186668 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MurNAc-LAA domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 326 amino acids
Theoretical weight: 36.65 KDa
Source organism: Listeria phage PSA
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8W5Y8 (Residues: 1-314; Coverage: 100%)
Gene name: ply
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand ZN 1 x ZN
Ligand SO4 1 x SO4
Ligand CL 1 x CL
Ligand GLU 1 x GLU
Ligand LYS 1 x LYS
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P6122
Unit cell:
a: 90.586Å b: 90.586Å c: 213.667Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.17 0.198
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Bacterial peptidoglycan (murein) hydrolases.
Vollmer et al. (2008)
12 more

15 mentions without citation

Structure-based modification of a Clostridium difficile-targeting endolysin affects activity and host range.
Mayer et al. (2011)
14 more