› 1xqe

X-ray diffraction
2.1Å resolution

The mechanism of ammonia transport based on the crystal structure of AmtB of E. coli.

Released:
DOI: 10.2210/pdb1xqe/pdb
PDB entry 1xqe coloured by chain, front view.
PDB entry 1xqe coloured by chain, side view.
PDB entry 1xqe coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The mechanism of ammonia transport based on the crystal structure of AmtB of Escherichia coli.
Zheng L, Kostrewa D, Bernèche S, Winkler FK, Li XD
Proc Natl Acad Sci U S A 101 17090-5 (2004)
PMID: 15563598

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159623 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ammonium transporter AmtB Chain: A
Molecule details ›
Chain: A
Length: 418 amino acids
Theoretical weight: 43.62 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69681 (Residues: 23-428; Coverage: 100%)
Gene names: JW0441, amtB, b0451, ybaG
Sequence domains: Ammonium Transporter Family
Structure domains: Ammonium transporter AmtB like domains

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R3
Unit cell:
a: 116.632Å b: 116.632Å c: 130.543Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.159 0.186
Expression system: Escherichia coli

Quick links

Citations

45 review citations

Getting across the cell membrane: an overview for small molecules, peptides, and proteins.
Yang et al. (2015)
44 more

8 mentions without citation

The mechanism of ammonia transport based on the crystal structure of AmtB of Escherichia coli.
Zheng et al. (2004)
7 more