› 1xrx

X-ray diffraction
2.15Å resolution

Crystal structure of a DNA-binding protein

Released:
DOI: 10.2210/pdb1xrx/pdb
PDB entry 1xrx coloured by chain, front view.
PDB entry 1xrx coloured by chain, side view.
PDB entry 1xrx coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of a SeqA-N filament: implications for DNA replication and chromosome organization.
Guarné A, Brendler T, Zhao Q, Ghirlando R, Austin S, Yang W
EMBO J 24 1502-11 (2005)
PMID: 15933720

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo octamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142769 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Negative modulator of initiation of replication Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 50 amino acids
Theoretical weight: 5.7 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFY8 (Residues: 1-50; Coverage: 28%)
Gene names: JW0674, b0687, seqA
Sequence domains: SeqA protein N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B, RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 112.179Å b: 112.179Å c: 62.021Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.222 0.237
Expression system: Escherichia coli

Quick links

Citations

11 review citations

DNA replication initiation: mechanisms and regulation in bacteria.
Mott et al. (2007)
10 more

4 mentions without citation

Bhageerath: an energy based web enabled computer software suite for limiting the search space of tertiary structures of small globular proteins.
Jayaram et al. (2006)
3 more