› 1xsb

Solution NMR

Structure of the nudix enzyme AP4A hydrolase from homo sapiens (E63A mutant) in complex with ATP. No ATP restraints included

Released:
DOI: 10.2210/pdb1xsb/pdb
PDB entry 1xsb coloured by chain, ensemble of 39 models, front view.
PDB entry 1xsb coloured by chain, ensemble of 39 models, side view.
PDB entry 1xsb coloured by chain, ensemble of 39 models, top view.
Source organism: Homo sapiens
Primary publication:
Structure and substrate-binding mechanism of human Ap4A hydrolase.
Swarbrick JD, Buyya S, Gunawardana D, Gayler KR, McLennan AG, Gooley PR
J Biol Chem 280 8471-81 (2005)
PMID: 15596429

Function and Biology Details

Reaction catalysed: 
P(1),P(4)-bis(5'-guanosyl) tetraphosphate + H(2)O = GTP + GMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156228 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical] Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 17.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P50583 (Residues: 1-147; Coverage: 100%)
Gene names: APAH1, NUDT2
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: CANDID xplor-NIH RAMA potential. Refine against CA & CB shifts.
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Dinucleoside polyphosphates in the eye: from physiology to therapeutics.
Guzmán-Aranguez et al. (2007)
1 more

1 mention without citation

Chlamydia trachomatis CT771 (nudH) is an asymmetric Ap4A hydrolase.
Barta et al. (2014)