› 1xtj

X-ray diffraction
2.7Å resolution

structure of human UAP56 in complex with ADP

Released:
DOI: 10.2210/pdb1xtj/pdb
PDB entry 1xtj coloured by chain, front view.
PDB entry 1xtj coloured by chain, side view.
PDB entry 1xtj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the human ATP-dependent splicing and export factor UAP56.
Shi H, Cordin O, Minder CM, Linder P, Xu RM
Proc Natl Acad Sci U S A 101 17628-33 (2004)
PMID: 15585580

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171824 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spliceosome RNA helicase DDX39B Chain: A
Molecule details ›
Chain: A
Length: 386 amino acids
Theoretical weight: 44.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13838 (Residues: 44-423; Coverage: 89%)
Gene names: BAT1, DDX39B, UAP56
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand ADP 1 x ADP
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P21
Unit cell:
a: 36.988Å b: 78.201Å c: 63.192Å
α: 90° β: 103.42° γ: 90°
R-values:
R R work R free
0.21 0.21 0.3
Expression system: Escherichia coli

Quick links

Citations

20 review citations

The DEAD-box protein family of RNA helicases.
Cordin et al. (2006)
19 more

10 mentions without citation

DEAD-box helicases as integrators of RNA, nucleotide and protein binding.
Putnam et al. (2013)
9 more