› 1y0e

X-ray diffraction
1.95Å resolution

Crystal structure of putative ManNAc-6-P epimerase from Staphylococcus aureus (strain N315)

Released:
DOI: 10.2210/pdb1y0e/pdb
PDB entry 1y0e coloured by chain, front view.
PDB entry 1y0e coloured by chain, side view.
PDB entry 1y0e coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus N315
Entry authors: Chang C, Joachimiak A, Li H, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
N-acyl-D-glucosamine 6-phosphate = N-acyl-D-mannosamine 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-159164 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative N-acetylmannosamine-6-phosphate 2-epimerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 223 amino acids
Theoretical weight: 24.96 KDa
Source organism: Staphylococcus aureus subsp. aureus N315
Expression system: Escherichia coli
UniProt:
  • Canonical: P65517 (Residues: 1-222; Coverage: 100%)
Gene names: SA0307, nanE
Sequence domains: Putative N-acetylmannosamine-6-phosphate epimerase
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand PO4 3 x PO4
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 52.961Å b: 76.283Å c: 183.661Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.216
Expression system: Escherichia coli

Quick links