› 1y14

X-ray diffraction
2.3Å resolution

Crystal structure of yeast subcomplex of Rpb4 and Rpb7

Released:
DOI: 10.2210/pdb1y14/pdb
PDB entry 1y14 coloured by chain, front view.
PDB entry 1y14 coloured by chain, side view.
PDB entry 1y14 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structures of complete RNA polymerase II and its subcomplex, Rpb4/7.
Armache KJ, Mitterweger S, Meinhart A, Cramer P
J Biol Chem 280 7131-4 (2005)
PMID: 15591044

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148972 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA-directed RNA polymerase II subunit RPB4 Chains: A, C
Molecule details ›
Chains: A, C
Length: 187 amino acids
Theoretical weight: 21.44 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P20433 (Residues: 35-221; Coverage: 85%)
Gene names: J0654, RPB4, YJL140W
Sequence domains: RNA polymerase Rpb4
Structure domains: Growth Hormone; Chain: A;
DNA-directed RNA polymerase II subunit RPB7 Chains: B, D
Molecule details ›
Chains: B, D
Length: 171 amino acids
Theoretical weight: 19.08 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P34087 (Residues: 1-171; Coverage: 100%)
Gene names: D9509.22, RPB7, YDR404C
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 103.646Å b: 114.81Å c: 80.479Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.274
Expression system: Escherichia coli

Quick links

Citations

35 review citations

The general transcription machinery and general cofactors.
Thomas et al. (2006)
34 more

4 mentions without citation

Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II.
Meka et al. (2005)
3 more