› 1y1o

X-ray diffraction
2.2Å resolution

X-ray crystal Structure of Penicillin-binding protein-related factor A from Bacillus stearothermophilus

Released:
DOI: 10.2210/pdb1y1o/pdb
PDB entry 1y1o coloured by chain, front view.
PDB entry 1y1o coloured by chain, side view.
PDB entry 1y1o coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Entry authors: Osipiuk J, Li H, Moy S, Collart F, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177380 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Holliday junction resolvase RecU Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 203 amino acids
Theoretical weight: 23.44 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5KXY4 (Residues: 1-200; Coverage: 100%)
Gene names: GK2167, recU
Sequence domains: Recombination protein U
Structure domains: Trna Endonuclease; Chain: A, domain 1

Ligands and Environments

2 bound ligands:
Ligand SO4 11 x SO4
Ligand NI 2 x NI
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 80.261Å b: 81.018Å c: 106.279Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.248
Expression system: Escherichia coli BL21(DE3)

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