› 1y9a

X-ray diffraction
1.81Å resolution

Alcohol Dehydrogenase from Entamoeba histolotica in complex with cacodylate

Released:
DOI: 10.2210/pdb1y9a/pdb
PDB entry 1y9a coloured by chain, front view.
PDB entry 1y9a coloured by chain, side view.
PDB entry 1y9a coloured by chain, top view.
Source organism: Entamoeba histolytica
Primary publication:
Structure of alcohol dehydrogenase from Entamoeba histolytica.
Shimon LJ, Goihberg E, Peretz M, Burstein Y, Frolow F
Acta Crystallogr D Biol Crystallogr 62 541-7 (2006)
PMID: 16627948

Function and Biology Details

Reaction catalysed: 
Propan-2-ol + NADP(+) = acetone + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152976 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent isopropanol dehydrogenase Chains: A, C
Molecule details ›
Chains: A, C
Length: 360 amino acids
Theoretical weight: 38.7 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
UniProt:
  • Canonical: P35630 (Residues: 7-366; Coverage: 98%)
Gene names: ADH1, EHI_023110
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand ZN 4 x ZN
Ligand CAC 2 x CAC
Ligand ACT 2 x ACT
Ligand MG 2 x MG
Ligand EDO 5 x EDO
1 modified residue:
Ligand OHS 2 x OHS

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2221
Unit cell:
a: 76.891Å b: 234.139Å c: 96.241Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.129 0.126 0.177
Expression system: Escherichia coli

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Citations

1 review citation

Toxicology and the biological role of methanol and ethanol: Current view.
Pohanka M. (2016)
4 other articles