› 1ycf

X-ray diffraction
3Å resolution

Oxidized (di-ferric) FprA from Moorella thermoacetica

Released:
DOI: 10.2210/pdb1ycf/pdb
PDB entry 1ycf coloured by chain, front view.
PDB entry 1ycf coloured by chain, side view.
PDB entry 1ycf coloured by chain, top view.
Source organism: Moorella thermoacetica
Primary publication:
X-ray crystal structures of Moorella thermoacetica FprA. Novel diiron site structure and mechanistic insights into a scavenging nitric oxide reductase.
Silaghi-Dumitrescu R, Kurtz DM, Ljungdahl LG, Lanzilotta WN
Biochemistry 44 6492-501 (2005)
PMID: 15850383

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-190300 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 398 amino acids
Theoretical weight: 44.22 KDa
Source organism: Moorella thermoacetica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9FDN7 (Residues: 2-399; Coverage: 100%)
Gene names: Moth_1287, fprA
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 3 x ZN
Ligand FEO 4 x FEO
Ligand OXY 4 x OXY
Ligand FMN 4 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P43212
Unit cell:
a: 159.67Å b: 159.67Å c: 276.798Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.26
Expression system: Escherichia coli

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Citations

11 review citations

Metallo-beta-lactamases (classification, activity, genetic organization, structure, zinc coordination) and their superfamily.
Bebrone C. (2007)
10 more

6 mentions without citation

Biological and Bioinspired Inorganic N-N Bond-Forming Reactions.
Ferousi et al. (2020)
5 more