› 1ycg

X-ray diffraction
2.8Å resolution

X-ray Structures of Moorella thermoacetica FprA. Novel Diiron Site Structure and Mechanistic Insights into a Scavenging Nitric Oxide Reductase

Released:
DOI: 10.2210/pdb1ycg/pdb
PDB entry 1ycg coloured by chain, front view.
PDB entry 1ycg coloured by chain, side view.
PDB entry 1ycg coloured by chain, top view.
Source organism: Moorella thermoacetica
Primary publication:
X-ray crystal structures of Moorella thermoacetica FprA. Novel diiron site structure and mechanistic insights into a scavenging nitric oxide reductase.
Silaghi-Dumitrescu R, Kurtz DM, Ljungdahl LG, Lanzilotta WN
Biochemistry 44 6492-501 (2005)
PMID: 15850383

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo octamer
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-190300 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 398 amino acids
Theoretical weight: 44.22 KDa
Source organism: Moorella thermoacetica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9FDN7 (Residues: 2-399; Coverage: 100%)
Gene names: Moth_1287, fprA
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 14 x ZN
Ligand FEO 4 x FEO
Ligand EDO 4 x EDO
Ligand FMN 4 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P43212
Unit cell:
a: 159.68Å b: 159.68Å c: 278.136Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.245
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Metallo-beta-lactamases (classification, activity, genetic organization, structure, zinc coordination) and their superfamily.
Bebrone C. (2007)
10 more

1 mention without citation

Spectroscopy and DFT Calculations of a Flavo-diiron Enzyme Implicate New Diiron Site Structures.
Weitz et al. (2017)