› 1yd8

X-ray diffraction
2.8Å resolution

COMPLEX OF HUMAN GGA3 GAT DOMAIN AND UBIQUITIN

Released:
DOI: 10.2210/pdb1yd8/pdb
PDB entry 1yd8 coloured by chain, front view.
PDB entry 1yd8 coloured by chain, side view.
PDB entry 1yd8 coloured by chain, top view.
Source organisms:
Primary publication:
Structural mechanism for ubiquitinated-cargo recognition by the Golgi-localized, gamma-ear-containing, ADP-ribosylation-factor-binding proteins.
Prag G, Lee S, Mattera R, Arighi CN, Beach BM, Bonifacino JS, Hurley JH
Proc Natl Acad Sci U S A 102 2334-9 (2005)
PMID: 15701688

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143488 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin Chains: U, V
Molecule details ›
Chains: U, V
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P0CH28 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
ADP-ribosylation factor-binding protein GGA3 Chains: G, H
Molecule details ›
Chains: G, H
Length: 98 amino acids
Theoretical weight: 11.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NZ52 (Residues: 208-301; Coverage: 13%)
Gene names: GGA3, KIAA0154
Sequence domains: GAT domain
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 47.886Å b: 97.293Å c: 66.459Å
α: 90° β: 90.03° γ: 90°
R-values:
R R work R free
0.221 0.221 0.297
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Ubiquitin-binding domains.
Hicke et al. (2005)
9 more

9 mentions without citation

Ubiquitin-binding domains.
Hurley et al. (2006)
8 more