› 1yee

X-ray diffraction
2.2Å resolution

STRUCTURE OF A CATALYTIC ANTIBODY, IGG2A FAB FRAGMENT (D2.5)

Released:
DOI: 10.2210/pdb1yee/pdb
PDB entry 1yee coloured by chain, front view.
PDB entry 1yee coloured by chain, side view.
PDB entry 1yee coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural convergence in the active sites of a family of catalytic antibodies.
Charbonnier JB, Golinelli-Pimpaneau B, Gigant B, Tawfik DS, Chap R, Schindler DG, Kim SH, Green BS, Eshhar Z, Knossow M
Science 275 1140-2 (1997)
PMID: 9027317

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-224993 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
IGG2A FAB FRAGMENT (D2.5) Chain: L
Molecule details ›
Chain: L
Length: 219 amino acids
Theoretical weight: 24.07 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Ig gamma-2A chain C region, membrane-bound form Chain: H
Molecule details ›
Chain: H
Length: 222 amino acids
Theoretical weight: 23.96 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01865 (Residues: 1-98; Coverage: 25%)
Gene name: Igh-1a
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand PNB 1 x PNB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE D41A
Spacegroup: P21
Unit cell:
a: 67.93Å b: 76.98Å c: 45.8Å
α: 90° β: 94.68° γ: 90°
R-values:
R R work R free
0.204 0.204 0.26

Quick links

Citations

8 review citations

Critical analysis of antibody catalysis.
Hilvert D. (2000)
7 more

2 mentions without citation

How to do an evaluation: pitfalls and traps.
Hawkins et al. (2008)
1 more