› 1ygw

Solution NMR

NMR STRUCTURE OF RIBONUCLEASE T1, 34 STRUCTURES

Released:
DOI: 10.2210/pdb1ygw/pdb
PDB entry 1ygw coloured by chain, ensemble of 34 models, front view.
PDB entry 1ygw coloured by chain, ensemble of 34 models, side view.
PDB entry 1ygw coloured by chain, ensemble of 34 models, top view.
Source organism: Aspergillus oryzae
Primary publication:
Limits of NMR structure determination using variable target function calculations: ribonuclease T1, a case study.
Pfeiffer S, Karimi-Nejad Y, Rüterjans H
J Mol Biol 266 400-23 (1997)
PMID: 9047372

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease T1 Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.09 KDa
Source organism: Aspergillus oryzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P00651 (Residues: 27-130; Coverage: 100%)
Gene names: AO090011000118, rntA
Structure domains: Microbial ribonucleases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: VARIABLE TARGET FUNCTION
Expression system: Escherichia coli

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Citations

2 review citations

Fungal ribotoxins: molecular dissection of a family of natural killers.
Lacadena et al. (2007)
1 more

9 mentions without citation

Search for allosteric disulfide bonds in NMR structures.
Schmidt et al. (2007)
8 more