› 1yis

X-ray diffraction
2.4Å resolution

Structural genomics of Caenorhabditis elegans: adenylosuccinate lyase

Released:
DOI: 10.2210/pdb1yis/pdb
PDB entry 1yis coloured by chain, front view.
PDB entry 1yis coloured by chain, side view.
PDB entry 1yis coloured by chain, top view.
Source organism: Caenorhabditis elegans
Entry authors: Symersky J, Schormann N, Lu S, Zhang Y, Karpova E, Qiu S, Huang W, Cao Z, Zhou J, Luo M, Arabshahi A, McKinstry A, Luan C-H, Luo D, Johnson D, An J, Tsao J, Delucas L, Shang Q, Gray R, Li S, Bray T, Chen Y-J, Southeast Collaboratory for Structural Genomics (SECSG)

Function and Biology Details

Reaction catalysed: 
(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173135 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate lyase Chain: A
Molecule details ›
Chain: A
Length: 478 amino acids
Theoretical weight: 54.2 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q21774 (Residues: 1-478; Coverage: 100%)
Gene names: R06C7.5, adsl-1
Sequence domains: Lyase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I422
Unit cell:
a: 124.242Å b: 124.242Å c: 165.127Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.174 0.202
Expression system: Escherichia coli

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