› 1ykf

X-ray diffraction
2.5Å resolution

Function and Biology Details

Reaction catalysed: 
Propan-2-ol + NADP(+) = acetone + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147162 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent isopropanol dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 352 amino acids
Theoretical weight: 37.69 KDa
Source organism: Thermoanaerobacter brockii
Expression system: Escherichia coli
UniProt:
  • Canonical: P14941 (Residues: 1-352; Coverage: 100%)
Gene name: adh
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P65
Unit cell:
a: 80.614Å b: 80.614Å c: 400.335Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.215 0.267
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Conformational changes and catalysis by alcohol dehydrogenase.
Plapp BV. (2010)
5 more

15 mentions without citation

Active site dynamics in the zinc-dependent medium chain alcohol dehydrogenase superfamily.
Baker et al. (2009)
14 more