› 1ypx

X-ray diffraction
2.6Å resolution

Crystal Structure of the Putative Vitamin-B12 Independent Methionine Synthase from Listeria monocytogenes, Northeast Structural Genomics Target LmR13

Released:
DOI: 10.2210/pdb1ypx/pdb
PDB entry 1ypx coloured by chain, front view.
PDB entry 1ypx coloured by chain, side view.
PDB entry 1ypx coloured by chain, top view.
Source organism: Listeria monocytogenes EGD-e
Entry authors: Forouhar F, Chen Y, Benach J, Xiao R, Acton TB, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187006 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cobalamin-independent methionine synthase MetE C-terminal/archaeal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 43.11 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8Y8Q1 (Residues: 1-367; Coverage: 100%)
Gene name: lmo0845
Sequence domains: Cobalamin-independent synthase, Catalytic domain
Structure domains: TIM Barrel

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: I41
Unit cell:
a: 139.109Å b: 139.109Å c: 38.535Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.233 0.308
Expression system: Escherichia coli

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