› 1yro

X-ray diffraction
1.9Å resolution

Crystal structure of beta14,-galactosyltransferase mutant ARG228Lys in complex with alpha-lactalbumin in the presence of UDP-galactose and Mn

Released:
DOI: 10.2210/pdb1yro/pdb
PDB entry 1yro coloured by chain, front view.
PDB entry 1yro coloured by chain, side view.
PDB entry 1yro coloured by chain, top view.
Source organisms:
Primary publication:
Mutation of arginine 228 to lysine enhances the glucosyltransferase activity of bovine beta-1,4-galactosyltransferase I.
Ramakrishnan B, Boeggeman E, Qasba PK
Biochemistry 44 3202-10 (2005)
PMID: 15736931

Function and Biology Details

Reactions catalysed: 
UDP-alpha-D-galactose + D-glucose = UDP + lactose
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139893 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Alpha-lactalbumin Chains: A, C
Molecule details ›
Chains: A, C
Length: 123 amino acids
Theoretical weight: 14.02 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P29752 (Residues: 21-143; Coverage: 100%)
Gene name: Lalba
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme
Beta-1,4-galactosyltransferase 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 286 amino acids
Theoretical weight: 32.82 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P08037 (Residues: 130-402; Coverage: 68%)
Gene names: B4GALT1, GALT, GGTB2
Sequence domains:
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

6 bound ligands:
Ligand CA 2 x CA
Ligand MES 1 x MES
Ligand PG4 2 x PG4
Ligand GDU 2 x GDU
Ligand MN 2 x MN
Ligand UDP 2 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P21
Unit cell:
a: 57.334Å b: 94.087Å c: 99.705Å
α: 90° β: 101.64° γ: 90°
R-values:
R R work R free
0.189 0.189 0.225
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

Substrate specificity of plant UDP-dependent glycosyltransferases predicted from crystal structures and homology modeling.
Osmani et al. (2009)
5 more

3 mentions without citation

Plumbagin elicits differential proteomic responses mainly involving cell cycle, apoptosis, autophagy, and epithelial-to-mesenchymal transition pathways in human prostate cancer PC-3 and DU145 cells.
Qiu et al. (2015)
2 more