› 1yta

X-ray diffraction
2.2Å resolution

Crystal Structure of Oligoribonuclease, the lone essential exoribonuclease in Escherichia coli

Released:
DOI: 10.2210/pdb1yta/pdb
PDB entry 1yta coloured by chain, front view.
PDB entry 1yta coloured by chain, side view.
PDB entry 1yta coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Fiedler TJ, Zuo Y, Malhotra A

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141551 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oligoribonuclease Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 180 amino acids
Theoretical weight: 20.94 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A784 (Residues: 2-181; Coverage: 99%)
Gene names: JW5740, b4162, o204a, orn, yjeR
Sequence domains: Exonuclease
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

1 bound ligand:
Ligand FLC 5 x FLC
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 70.43Å b: 72.87Å c: 147.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.249
Expression system: Escherichia coli BL21(DE3)

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