› 1yuo

X-ray diffraction
1.95Å resolution

Optimisation of the surface electrostatics as a strategy for cold adaptation of uracil-DNA N-glycosylase (UNG)from atlantic cod (Gadus morhua)

Released:
DOI: 10.2210/pdb1yuo/pdb
PDB entry 1yuo coloured by chain, front view.
PDB entry 1yuo coloured by chain, side view.
PDB entry 1yuo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Optimisation of the surface electrostatics as a strategy for cold adaptation of uracil-DNA N-glycosylase (UNG) from Atlantic cod (Gadus morhua).
Moe E, Leiros I, Riise EK, Olufsen M, Lanes O, Smalås A, Willassen NP
J Mol Biol 343 1221-30 (2004)
PMID: 15491608

Function and Biology Details

Reaction catalysed: 
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uracil-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 25.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13051 (Residues: 91-313; Coverage: 71%)
Gene names: DGU, UNG, UNG1, UNG15
Sequence domains: Uracil DNA glycosylase superfamily
Structure domains: Uracil-DNA glycosylase-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM1A
Spacegroup: P212121
Unit cell:
a: 47.511Å b: 54.771Å c: 78.071Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.254
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Cold-adapted enzymes.
Siddiqui et al. (2006)
2 more