› 1yw4

X-ray diffraction
2Å resolution

Crystal Structure of the Succinylglutamate Desuccinylase from Chromobacterium violaceum, Northeast Structural Genomics Target CvR22.

Released:
DOI: 10.2210/pdb1yw4/pdb
PDB entry 1yw4 coloured by chain, front view.
PDB entry 1yw4 coloured by chain, side view.
PDB entry 1yw4 coloured by chain, top view.
Source organism: Chromobacterium violaceum ATCC 12472
Entry authors: Forouhar F, Abashidze M, Conover K, Acton TB, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
N-succinyl-L-glutamate + H(2)O = succinate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181834 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Succinylglutamate desuccinylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 341 amino acids
Theoretical weight: 37.87 KDa
Source organism: Chromobacterium violaceum ATCC 12472
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7NU26 (Residues: 1-333; Coverage: 100%)
Gene names: CV_2877, astE
Sequence domains: Succinylglutamate desuccinylase / Aspartoacylase family
Structure domains: Zn peptidases

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 62.95Å b: 39.435Å c: 131.585Å
α: 90° β: 94.74° γ: 90°
R-values:
R R work R free
0.224 0.221 0.262
Expression system: Escherichia coli

Quick links