› 1yx2

X-ray diffraction
2.08Å resolution

Crystal Structure of the Probable Aminomethyltransferase from Bacillus subtilis

Released:
DOI: 10.2210/pdb1yx2/pdb
PDB entry 1yx2 coloured by chain, front view.
PDB entry 1yx2 coloured by chain, side view.
PDB entry 1yx2 coloured by chain, top view.
Source organism: Bacillus subtilis
Entry author: Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157018 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminomethyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 365 amino acids
Theoretical weight: 40.45 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54378 (Residues: 1-362; Coverage: 100%)
Gene names: BSU24570, gcvT, yqhI
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 47.628Å b: 88.095Å c: 88.764Å
α: 90° β: 101.78° γ: 90°
R-values:
R R work R free
0.219 0.212 0.288
Expression system: Escherichia coli BL21(DE3)

Quick links