› 1yxo

X-ray diffraction
2.01Å resolution

Crystal Structure of pyridoxal phosphate biosynthetic protein PdxA PA0593

Released:
DOI: 10.2210/pdb1yxo/pdb
PDB entry 1yxo coloured by chain, front view.
PDB entry 1yxo coloured by chain, side view.
PDB entry 1yxo coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Entry authors: Liu Y, Xu X, Dong A, Kudritskam M, Savchenko A, Pai EF, Joachimiak A, Edwards A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
4-phosphooxy-L-threonine + NAD(+) = 3-amino-2-oxopropyl phosphate + CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191429 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxythreonine-4-phosphate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 328 amino acids
Theoretical weight: 35.25 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I5U4 (Residues: 1-328; Coverage: 100%)
Gene names: PA0593, pdxA
Sequence domains: Pyridoxal phosphate biosynthetic protein PdxA
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand EOH 10 x EOH
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P212121
Unit cell:
a: 54.259Å b: 59.307Å c: 199.111Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.243
Expression system: Escherichia coli BL21(DE3)

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