› 1yyw

X-ray diffraction
2.8Å resolution

Crystal structure of RNase III from Aquifex aeolicus complexed with double stranded RNA at 2.8-Angstrom Resolution

Released:
DOI: 10.2210/pdb1yyw/pdb
PDB entry 1yyw coloured by chain, front view.
PDB entry 1yyw coloured by chain, side view.
PDB entry 1yyw coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Intermediate states of ribonuclease III in complex with double-stranded RNA.
Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X
Structure 13 1435-42 (2005)
PMID: 16216575

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130433 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Ribonuclease 3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 221 amino acids
Theoretical weight: 26.15 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67082 (Residues: 1-221; Coverage: 100%)
Gene names: aq_946, rnc
Sequence domains:
Structure domains:
5'-R(*AP*AP*AP*UP*AP*UP*AP*UP*AP*UP*UP*U)-3' Chains: E, F, G, H, I, J, K, L
Molecule details ›
Chains: E, F, G, H, I, J, K, L
Length: 12 nucleotides
Theoretical weight: 3.77 KDa
Source organism: Aquifex aeolicus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 86.428Å b: 95.906Å c: 166.004Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.243 0.314
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Ribonuclease revisited: structural insights into ribonuclease III family enzymes.
MacRae et al. (2007)
4 more

7 mentions without citation

RNA recognition by double-stranded RNA binding domains: a matter of shape and sequence.
Masliah et al. (2013)
6 more