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Solution NMR

Solution structure of the Josephin domain of Ataxin-3

Released:

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156965 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ataxin-3 Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 21.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54252 (Residues: 1-182; Coverage: 50%)
Gene names: ATX3, ATXN3, MJD, MJD1, SCA3
Sequence domains: Josephin
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)