› 1z17

X-ray diffraction
1.96Å resolution

Crystal structure analysis of periplasmic Leu/Ile/Val-binding protein with bound ligand isoleucine

Released:
DOI: 10.2210/pdb1z17/pdb
PDB entry 1z17 coloured by chain, front view.
PDB entry 1z17 coloured by chain, side view.
PDB entry 1z17 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Ligand-free and -bound structures of the binding protein (LivJ) of the Escherichia coli ABC leucine/isoleucine/valine transport system: trajectory and dynamics of the interdomain rotation and ligand specificity.
Trakhanov S, Vyas NK, Luecke H, Kristensen DM, Ma J, Quiocho FA
Biochemistry 44 6597-608 (2005)
PMID: 15850393

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142337 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leu/Ile/Val-binding protein Chain: A
Molecule details ›
Chain: A
Length: 344 amino acids
Theoretical weight: 36.81 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0AD96 (Residues: 24-367; Coverage: 100%)
Gene names: JW3425, b3460, livJ
Sequence domains: Periplasmic binding protein
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand ILE 1 x ILE
Ligand MRD 1 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 99.13Å b: 99.13Å c: 93.18Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.183 0.205

Quick links

Citations

11 review citations

Structure, function, and evolution of bacterial ATP-binding cassette systems.
Davidson et al. (2008)
10 more

6 mentions without citation

A conserved mechanism of GABA binding and antagonism is revealed by structure-function analysis of the periplasmic binding protein Atu2422 in Agrobacterium tumefaciens.
Planamente et al. (2010)
5 more