› 1z1l

X-ray diffraction
1.7Å resolution

The Crystal Structure of the Phosphodiesterase 2A Catalytic Domain

Released:
DOI: 10.2210/pdb1z1l/pdb
PDB entry 1z1l coloured by chain, front view.
PDB entry 1z1l coloured by chain, side view.
PDB entry 1z1l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural determinants for inhibitor specificity and selectivity in PDE2A using the wheat germ in vitro translation system.
Iffland A, Kohls D, Low S, Luan J, Zhang Y, Kothe M, Cao Q, Kamath AV, Ding YH, Ellenberger T
Biochemistry 44 8312-25 (2005)
PMID: 15938621

Function and Biology Details

Reaction catalysed: 
Nucleoside 3',5'-cyclic phosphate + H(2)O = nucleoside 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126260 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cGMP-dependent 3',5'-cyclic phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 345 amino acids
Theoretical weight: 40.31 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O00408 (Residues: 575-919; Coverage: 37%)
Gene name: PDE2A
Sequence domains: 3'5'-cyclic nucleotide phosphodiesterase
Structure domains: 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand MG 1 x MG
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2221
Unit cell:
a: 90.033Å b: 102.549Å c: 81.598Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.237
Expression system: Spodoptera frugiperda

Quick links

Citations

6 review citations

Mammalian cyclic nucleotide phosphodiesterases: molecular mechanisms and physiological functions.
Francis et al. (2011)
5 more

6 mentions without citation

Sulindac sulfide selectively inhibits growth and induces apoptosis of human breast tumor cells by phosphodiesterase 5 inhibition, elevation of cyclic GMP, and activation of protein kinase G.
Tinsley et al. (2009)
5 more