› 1z2m

X-ray diffraction
2.5Å resolution

Crystal Structure of ISG15, the Interferon-Induced Ubiquitin Cross Reactive Protein

Released:
DOI: 10.2210/pdb1z2m/pdb
PDB entry 1z2m coloured by chain, front view.
PDB entry 1z2m coloured by chain, side view.
PDB entry 1z2m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the interferon-induced ubiquitin-like protein ISG15.
Narasimhan J, Wang M, Fu Z, Klein JM, Haas AL, Kim JJ
J Biol Chem 280 27356-65 (2005)
PMID: 15917233

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138564 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-like protein ISG15 Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 17.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05161 (Residues: 1-155; Coverage: 94%)
Gene names: G1P2, ISG15, UCRP
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand OS4 1 x OS4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I222
Unit cell:
a: 55.79Å b: 56.94Å c: 103.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

37 review citations

ISG15 in antiviral immunity and beyond.
Perng et al. (2018)
36 more

45 mentions without citation

Nsp3 of coronaviruses: Structures and functions of a large multi-domain protein.
Lei et al. (2018)
44 more