› 1z4w

X-ray diffraction
2.7Å resolution

Parainfluenza Virus 5 (SV5) Hemagglutinin-Neuraminidase (HN) with ligand DANA (soaked with DANA, pH8.0)

Released:
DOI: 10.2210/pdb1z4w/pdb
PDB entry 1z4w coloured by chain, front view.
PDB entry 1z4w coloured by chain, side view.
PDB entry 1z4w coloured by chain, top view.
Source organism: Mammalian orthorubulavirus 5
Primary publication:
Structural studies of the parainfluenza virus 5 hemagglutinin-neuraminidase tetramer in complex with its receptor, sialyllactose.
Yuan P, Thompson TB, Wurzburg BA, Paterson RG, Lamb RA, Jardetzky TS
Structure 13 803-15 (2005)
PMID: 15893670

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138212 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Hemagglutinin-neuraminidase Chain: A
Molecule details ›
Chain: A
Length: 532 amino acids
Theoretical weight: 58.51 KDa
Source organism: Mammalian orthorubulavirus 5
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P04850 (Residues: 37-565; Coverage: 94%)
Gene name: HN
Sequence domains: Haemagglutinin-neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand NAG 1 x NAG
Ligand CA 1 x CA
Ligand DAN 1 x DAN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: P6122
Unit cell:
a: 137.976Å b: 137.976Å c: 185.041Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.198 0.222
Expression system: Trichoplusia ni

Quick links

Citations

32 review citations

Structures and mechanisms of viral membrane fusion proteins: multiple variations on a common theme.
White et al. (2008)
31 more