› 1z5w

X-ray diffraction
3Å resolution

Crystal Structure of gamma-tubulin bound to GTP

Released:
DOI: 10.2210/pdb1z5w/pdb
PDB entry 1z5w coloured by chain, front view.
PDB entry 1z5w coloured by chain, side view.
PDB entry 1z5w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into microtubule nucleation from the crystal structure of human gamma-tubulin.
Aldaz H, Rice LM, Stearns T, Agard DA
Nature 435 523-7 (2005)
PMID: 15917813

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149909 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tubulin gamma-1 chain Chain: A
Molecule details ›
Chain: A
Length: 474 amino acids
Theoretical weight: 53.91 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P23258 (Residues: 1-449; Coverage: 100%)
Gene names: TUBG, TUBG1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GTP 1 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 52.19Å b: 76.29Å c: 64.81Å
α: 90° β: 102.31° γ: 90°
R-values:
R R work R free
0.254 0.247 0.309
Expression system: Spodoptera frugiperda

Quick links

Citations

22 review citations

Microtubule nucleation by γ-tubulin complexes.
Kollman et al. (2011)
21 more

7 mentions without citation

γ-Tubulin in microtubule nucleation and beyond.
Sulimenko et al. (2022)
6 more