› 1z7n

X-ray diffraction
3.25Å resolution

ATP Phosphoribosyl transferase (HisZG ATP-PRTase) from Lactococcus lactis with bound PRPP substrate

Released:
DOI: 10.2210/pdb1z7n/pdb
PDB entry 1z7n coloured by chain, front view.
PDB entry 1z7n coloured by chain, side view.
PDB entry 1z7n coloured by chain, top view.
Source organism: Lactococcus lactis
Primary publication:
Activation of the hetero-octameric ATP phosphoribosyl transferase through subunit interface rearrangement by a tRNA synthetase paralog.
Champagne KS, Sissler M, Larrabee Y, Doublié S, Francklyn CS
J Biol Chem 280 34096-104 (2005)
PMID: 16051603

Function and Biology Details

Reaction catalysed: 
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATP phosphoribosyltransferase regulatory subunit Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 344 amino acids
Theoretical weight: 39.85 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02147 (Residues: 1-328; Coverage: 100%)
Gene names: L0341, LL1207, hisZ
Sequence domains: Histidyl-tRNA synthetase
Structure domains: Bira Bifunctional Protein; Domain 2
ATP phosphoribosyltransferase Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 208 amino acids
Theoretical weight: 23.71 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02129 (Residues: 1-208; Coverage: 100%)
Gene names: L0066, LL1208, hisG
Sequence domains: ATP phosphoribosyltransferase
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand PRP 4 x PRP
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21212
Unit cell:
a: 151.684Å b: 222.936Å c: 86.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.298 0.247 0.301
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Phosphoribosyl Diphosphate (PRPP): Biosynthesis, Enzymology, Utilization, and Metabolic Significance.
Hove-Jensen et al. (2017)
1 more

3 mentions without citation

Structure, mechanism and inhibition of anthranilate phosphoribosyltransferase.
Scully et al. (2023)
2 more