› 1ze1

X-ray diffraction
2.9Å resolution

Conformational Change of Pseudouridine 55 Synthase upon Its Association with RNA Substrate

Released:
DOI: 10.2210/pdb1ze1/pdb
PDB entry 1ze1 coloured by chain, front view.
PDB entry 1ze1 coloured by chain, side view.
PDB entry 1ze1 coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Conformational change of pseudouridine 55 synthase upon its association with RNA substrate.
Phannachet K, Huang RH
Nucleic Acids Res 32 1422-9 (2004)
PMID: 14990747

Function and Biology Details

Reaction catalysed: 
tRNA uridine(55) = tRNA pseudouridine(55)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194644 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA pseudouridine synthase B Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 309 amino acids
Theoretical weight: 35.57 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WZW0 (Residues: 1-309; Coverage: 100%)
Gene names: TM_0856, truB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P1
Unit cell:
a: 44.65Å b: 64.17Å c: 176.07Å
α: 87.48° β: 86.44° γ: 79.35°
R-values:
R R work R free
0.235 0.235 0.281
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Pseudouridine synthases.
Hamma et al. (2006)
8 more

2 mentions without citation

Conformational changes in redox pairs of protein structures.
Fan et al. (2009)
1 more