› 1zgl

X-ray diffraction
2.8Å resolution

Crystal structure of 3A6 TCR bound to MBP/HLA-DR2a

Released:
DOI: 10.2210/pdb1zgl/pdb
PDB entry 1zgl coloured by chain, front view.
PDB entry 1zgl coloured by chain, side view.
PDB entry 1zgl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a human autoimmune TCR bound to a myelin basic protein self-peptide and a multiple sclerosis-associated MHC class II molecule.
Li Y, Huang Y, Lue J, Quandt JA, Martin R, Mariuzza RA
EMBO J 24 2968-79 (2005)
PMID: 16079912

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-134727 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class II histocompatibility antigen, DR alpha chain Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 181 amino acids
Theoretical weight: 21.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01903 (Residues: 26-206; Coverage: 79%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DR beta 5 chain Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 192 amino acids
Theoretical weight: 22.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q30154 (Residues: 30-221; Coverage: 81%)
Gene name: HLA-DRB5
Sequence domains:
Structure domains:
Myelin basic protein Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 15 amino acids
Theoretical weight: 1.67 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P02686 (Residues: 221-233; Coverage: 4%)
Gene name: MBP
T cell receptor alpha chain constant Chains: M, Q, S, U
Molecule details ›
Chains: M, Q, S, U
Length: 209 amino acids
Theoretical weight: 22.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01848 (Residues: 1-94; Coverage: 67%)
Gene names: TCRA, TRAC
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins
T cell receptor beta constant 1 Chains: P, R, T, V
Molecule details ›
Chains: P, R, T, V
Length: 249 amino acids
Theoretical weight: 27.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01850 (Residues: 1-130; Coverage: 74%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P1
Unit cell:
a: 96.48Å b: 97.69Å c: 124.02Å
α: 74.39° β: 83.19° γ: 61.55°
R-values:
R R work R free
0.28 0.28 0.329
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

46 review citations

How TCRs bind MHCs, peptides, and coreceptors.
Rudolph et al. (2006)
45 more

35 mentions without citation

Recognition of self-peptide-MHC complexes by autoimmune T-cell receptors.
Deng et al. (2007)
34 more