› 1zgn

X-ray diffraction
2.1Å resolution

Crystal Structure of the Glutathione Transferase Pi in Complex with Dinitrosyl-diglutathionyl Iron Complex

Released:
DOI: 10.2210/pdb1zgn/pdb
PDB entry 1zgn coloured by chain, front view.
PDB entry 1zgn coloured by chain, side view.
PDB entry 1zgn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Nitrosylation of human glutathione transferase P1-1 with dinitrosyl diglutathionyl iron complex in vitro and in vivo.
Cesareo E, Parker LJ, Pedersen JZ, Nuccetelli M, Mazzetti AP, Pastore A, Federici G, Caccuri AM, Ricci G, Adams JJ, Parker MW, Lo Bello M
J Biol Chem 280 42172-80 (2005)
PMID: 16195232

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140593 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase P Chains: A, B
Molecule details ›
Chains: A, B
Length: 209 amino acids
Theoretical weight: 23.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09211 (Residues: 2-210; Coverage: 100%)
Gene names: FAEES3, GST3, GSTP1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 2 x GSH
3 bound ligands:
Ligand FE 2 x FE
Ligand MES 2 x MES
Ligand NO 4 x NO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2
Unit cell:
a: 76.219Å b: 89.759Å c: 68.713Å
α: 90° β: 97.632° γ: 90°
R-values:
R R work R free
0.182 0.182 0.244
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Glutathione transferases and development of new principles to overcome drug resistance.
Sau et al. (2010)
10 more

4 mentions without citation

Glutathione Transferase P1-1 an Enzyme Useful in Biomedicine and as Biomarker in Clinical Practice and in Environmental Pollution.
Bocedi et al. (2019)
3 more