› 1zki

X-ray diffraction
1.7Å resolution

Structure of conserved protein PA5202 from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb1zki/pdb
PDB entry 1zki coloured by chain, front view.
PDB entry 1zki coloured by chain, side view.
PDB entry 1zki coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structure and activity of the Pseudomonas aeruginosa hotdog-fold thioesterases PA5202 and PA2801.
Gonzalez CF, Tchigvintsev A, Brown G, Flick R, Evdokimova E, Xu X, Osipiuk J, Cuff ME, Lynch S, Joachimiak A, Savchenko A, Yakunin AF
Biochem J 444 445-55 (2012)
PMID: 22439787

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190940 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Medium/long-chain acyl-CoA thioesterase YigI Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 14.48 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9HTY7 (Residues: 1-129; Coverage: 100%)
Gene name: PA5202
Sequence domains: Thioesterase superfamily
Structure domains: Hotdog Thioesterase

Ligands and Environments

1 bound ligand:
Ligand ACY 1 x ACY
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P3121
Unit cell:
a: 57.948Å b: 57.948Å c: 113.502Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.16 0.194
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Enzyme promiscuity: engine of evolutionary innovation.
Pandya et al. (2014)
4 other articles

2 mentions without citation

Analysis of proteins with the 'hot dog' fold: prediction of function and identification of catalytic residues of hypothetical proteins.
Pidugu et al. (2009)
1 more