› 1zmd

X-ray diffraction
2.08Å resolution

Crystal Structure of Human dihydrolipoamide dehydrogenase complexed to NADH

Released:
DOI: 10.2210/pdb1zmd/pdb
PDB entry 1zmd coloured by chain, front view.
PDB entry 1zmd coloured by chain, side view.
PDB entry 1zmd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human dihydrolipoamide dehydrogenase: NAD+/NADH binding and the structural basis of disease-causing mutations.
Brautigam CA, Chuang JL, Tomchick DR, Machius M, Chuang DT
J Mol Biol 350 543-52 (2005)
PMID: 15946682

Function and Biology Details

Reaction catalysed: 
Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140717 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrolipoyl dehydrogenase, mitochondrial Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 474 amino acids
Theoretical weight: 50.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09622 (Residues: 36-509; Coverage: 93%)
Gene names: DLD, GCSL, LAD, PHE3
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAI 8 x NAI

Cofactor: Ligand FAD 8 x FAD
1 bound ligand:
Ligand SO4 31 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 175.871Å b: 210.914Å c: 127.096Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.249
Expression system: Escherichia coli

Quick links

Citations

12 review citations

The pyruvate dehydrogenase complexes: structure-based function and regulation.
Patel et al. (2014)
11 more

8 mentions without citation

Triazaspirodimethoxybenzoyls as selective inhibitors of mycobacterial lipoamide dehydrogenase .
Bryk et al. (2010)
7 more