› 1zn9

X-ray diffraction
2.05Å resolution

Human Adenine Phosphoribosyltransferase in Apo and AMP Complexed Forms

Released:
DOI: 10.2210/pdb1zn9/pdb
PDB entry 1zn9 coloured by chain, front view.
PDB entry 1zn9 coloured by chain, side view.
PDB entry 1zn9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural complexes of human adenine phosphoribosyltransferase reveal novel features of the APRT catalytic mechanism.
Silva CH, Silva M, Iulek J, Thiemann OH
J Biomol Struct Dyn 25 589-97 (2008)
PMID: 18399692

Function and Biology Details

Reaction catalysed: 
AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139738 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenine phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 180 amino acids
Theoretical weight: 19.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07741 (Residues: 1-180; Coverage: 100%)
Gene name: APRT
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 47.511Å b: 49.252Å c: 140.105Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.151 0.225
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

From purines to purinergic signalling: molecular functions and human diseases.
Huang et al. (2021)
1 more

1 mention without citation

Molecular docking investigation of the amantadine binding to the enzymes upregulated or downregulated in Parkinson's disease.
Ionescu MI. (2020)