› 1zrt

X-ray diffraction
3.51Å resolution

Rhodobacter capsulatus cytochrome bc1 complex with stigmatellin bound

Released:
DOI: 10.2210/pdb1zrt/pdb
PDB entry 1zrt coloured by chain, front view.
PDB entry 1zrt coloured by chain, side view.
PDB entry 1zrt coloured by chain, top view.
Source organism: Rhodobacter capsulatus
Primary publication:
X-Ray Structure of Rhodobacter Capsulatus Cytochrome bc (1): Comparison with its Mitochondrial and Chloroplast Counterparts.
Berry EA, Huang LS, Saechao LK, Pon NG, Valkova-Valchanova M, Daldal F
Photosynth Res 81 251-75 (2004)
PMID: 16034531

Function and Biology Details

Reaction catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-143572 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cytochrome b Chains: C, P
Molecule details ›
Chains: C, P
Length: 437 amino acids
Theoretical weight: 49.39 KDa
Source organism: Rhodobacter capsulatus
Expression system: Rhodobacter capsulatus
UniProt:
  • Canonical: P0CY47 (Residues: 1-437; Coverage: 100%)
Gene names: cytB, petB
Sequence domains:
Structure domains: Cytochrome Bc1 Complex; Chain C
Cytochrome c1 Chains: D, Q
Molecule details ›
Chains: D, Q
Length: 258 amino acids
Theoretical weight: 28.32 KDa
Source organism: Rhodobacter capsulatus
Expression system: Rhodobacter capsulatus
UniProt:
  • Canonical: P0CY49 (Residues: 22-280; Coverage: 100%)
Gene name: petC
Sequence domains: Cytochrome C1 family
Ubiquinol-cytochrome c reductase iron-sulfur subunit Chains: E, R
Molecule details ›
Chains: E, R
Length: 191 amino acids
Theoretical weight: 20.47 KDa
Source organism: Rhodobacter capsulatus
Expression system: Rhodobacter capsulatus
UniProt:
  • Canonical: P0CY48 (Residues: 1-191; Coverage: 100%)
Gene names: fbcF, petA
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand HEM 4 x HEM
Ligand SMA 2 x SMA
Ligand UNL 4 x UNL
Ligand HEC 2 x HEC
Ligand FES 2 x FES
Ligand PG6 1 x PG6
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1, ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 95.633Å b: 154.36Å c: 103.057Å
α: 90° β: 113.57° γ: 90°
R-values:
R R work R free
0.224 0.221 0.289
Expression system: Rhodobacter capsulatus

Quick links

Citations

19 review citations

Superoxide generation by complex III: from mechanistic rationales to functional consequences.
Bleier et al. (2013)
18 more

35 mentions without citation

Structural analysis of cytochrome bc1 complexes: implications to the mechanism of function.
Xia et al. (2013)
34 more