1zt2

X-ray diffraction
3.33Å resolution

Heterodimeric structure of the core primase.

Released:
Source organism: Saccharolobus solfataricus
Primary publication:
Structure of the heterodimeric core primase.
Nat Struct Mol Biol 12 1137-44 (2005)
PMID: 16273105

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-189064 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA primase small subunit PriS Chains: A, C
Molecule details ›
Chains: A, C
Length: 330 amino acids
Theoretical weight: 37.64 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97Z83 (Residues: 1-330; Coverage: 100%)
Gene names: SSO1048, priA, priS
Sequence domains:
Structure domains: DNA primase, PRIM domain
DNA primase large subunit PriL Chains: B, D
Molecule details ›
Chains: B, D
Length: 212 amino acids
Theoretical weight: 24.91 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UWW1 (Residues: 1-212; Coverage: 69%)
Gene names: C21_042, SSO0557, priB, priL
Sequence domains: Eukaryotic and archaeal DNA primase, large subunit

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P42212
Unit cell:
a: 193.287Å b: 193.287Å c: 213.029Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.236 0.243
Expression system: Escherichia coli