› 1zum

X-ray diffraction
2.1Å resolution

Human Mitochondrial Aldehyde Dehydrogenase Asian Variant, ALDH2*2, Apo Form

Released:
DOI: 10.2210/pdb1zum/pdb
PDB entry 1zum coloured by chain, front view.
PDB entry 1zum coloured by chain, side view.
PDB entry 1zum coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Disruption of the coenzyme binding site and dimer interface revealed in the crystal structure of mitochondrial aldehyde dehydrogenase "Asian" variant.
Larson HN, Weiner H, Hurley TD
J Biol Chem 280 30550-6 (2005)
PMID: 15983043

Function and Biology Details

Reaction catalysed: 
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138420 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde dehydrogenase, mitochondrial Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 500 amino acids
Theoretical weight: 54.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P05091 (Residues: 18-517; Coverage: 97%)
Gene names: ALDH2, ALDM
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NA 12 x NA
Ligand GAI 8 x GAI
Ligand EDO 21 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 95.873Å b: 105.261Å c: 162.672Å
α: 78.81° β: 81.95° γ: 88.04°
R-values:
R R work R free
0.21 0.204 0.238
Expression system: Escherichia coli BL21

Quick links

Citations

22 review citations

Aldehyde dehydrogenase inhibitors: a comprehensive review of the pharmacology, mechanism of action, substrate specificity, and clinical application.
Koppaka et al. (2012)
21 more

8 mentions without citation

Targeting aldehyde dehydrogenase 2: new therapeutic opportunities.
Chen et al. (2014)
7 more