› 1zw6

X-ray diffraction
1.5Å resolution

Crystal Structure of the GTP-bound form of RasQ61G

Released:
DOI: 10.2210/pdb1zw6/pdb
PDB entry 1zw6 coloured by chain, front view.
PDB entry 1zw6 coloured by chain, side view.
PDB entry 1zw6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a transient intermediate for GTP hydrolysis by ras.
Ford B, Hornak V, Kleinman H, Nassar N
Structure 14 427-36 (2006)
PMID: 16531227

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 3 x MG
Ligand CA 2 x CA
Ligand GNP 1 x GNP
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: R32
Unit cell:
a: 88.776Å b: 88.776Å c: 134.392Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.146 0.144 0.174
Expression system: Escherichia coli

Quick links

Citations

5 review citations

CHARMM: the biomolecular simulation program.
Brooks et al. (2009)
4 more

5 mentions without citation

Transformation efficiency of RasQ61 mutants linked to structural features of the switch regions in the presence of Raf.
Buhrman et al. (2007)
4 more