› 1zy4

X-ray diffraction
1.95Å resolution

Crystal Structure of eIF2alpha Protein Kinase GCN2: R794G Hyperactivating Mutant in Apo Form.

Released:
DOI: 10.2210/pdb1zy4/pdb
PDB entry 1zy4 coloured by chain, front view.
PDB entry 1zy4 coloured by chain, side view.
PDB entry 1zy4 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis for autoinhibition and mutational activation of eukaryotic initiation factor 2alpha protein kinase GCN2.
Padyana AK, Qiu H, Roll-Mecak A, Hinnebusch AG, Burley SK
J Biol Chem 280 29289-99 (2005)
PMID: 15964839

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147365 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
eIF-2-alpha kinase GCN2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 303 amino acids
Theoretical weight: 35.04 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P15442 (Residues: 594-997; Coverage: 18%)
Gene names: AAS1, GCN2, YDR283C
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 52.833Å b: 79.257Å c: 146.863Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.252
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Eukaryotic initiation factor 2 phosphorylation and translational control in metabolism.
Baird et al. (2012)
19 more

6 mentions without citation

Intracellular eukaryotic parasites have a distinct unfolded protein response.
Gosline et al. (2011)
5 more