› 2a25

X-ray diffraction
2.2Å resolution

Crystal structure of Siah1 SBD bound to the peptide EKPAAVVAPITTG from SIP

Released:
DOI: 10.2210/pdb2a25/pdb
PDB entry 2a25 coloured by chain, front view.
PDB entry 2a25 coloured by chain, side view.
PDB entry 2a25 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of Siah1-Siah-interacting protein interactions and insights into the assembly of an E3 ligase multiprotein complex.
Santelli E, Leone M, Li C, Fukushima T, Preece NE, Olson AJ, Ely KR, Reed JC, Pellecchia M, Liddington RC, Matsuzawa S
J Biol Chem 280 34278-87 (2005)
PMID: 16085652

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184858 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase SIAH1 Chain: A
Molecule details ›
Chain: A
Length: 193 amino acids
Theoretical weight: 21.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IUQ4 (Residues: 90-282; Coverage: 68%)
Gene names: HUMSIAH, SIAH1
Sequence domains:
Structure domains:
Calcyclin-binding protein Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.25 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9HB71 (Residues: 58-70; Coverage: 6%)
Gene names: CACYBP, PNAS-107, S100A6BP, SIP

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E
Spacegroup: C2221
Unit cell:
a: 47.635Å b: 107.761Å c: 78.414Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.252
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

8 review citations

S100A6 binding protein and Siah-1 interacting protein (CacyBP/SIP): spotlight on properties and cellular function.
Schneider et al. (2011)
7 more

10 mentions without citation

The Siah2-HIF-FoxA2 axis in prostate cancer – new markers and therapeutic opportunities.
Qi et al. (2010)
9 more