› 2a30

X-ray diffraction
3.02Å resolution

Crystal structure of human deoxycytidine kinase in complex with deoxycytidine

Released:
DOI: 10.2210/pdb2a30/pdb
PDB entry 2a30 coloured by chain, front view.
PDB entry 2a30 coloured by chain, side view.
PDB entry 2a30 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the preference of UTP over ATP in human deoxycytidine kinase: illuminating the role of main-chain reorganization.
Godsey MH, Ort S, Sabini E, Konrad M, Lavie A
Biochemistry 45 452-61 (2006)
PMID: 16401075

Function and Biology Details

Reactions catalysed: 
ATP + deoxyguanosine = ADP + dGMP
NTP + deoxycytidine = NDP + dCMP
ATP + deoxyadenosine = ADP + dAMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151058 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxycytidine kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 248 amino acids
Theoretical weight: 29.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27707 (Residues: 1-260; Coverage: 94%)
Gene name: DCK
Sequence domains: Deoxynucleoside kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand DCZ 4 x DCZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 79.337Å b: 64.941Å c: 108.602Å
α: 90° β: 95.97° γ: 90°
R-values:
R R work R free
0.24 0.235 0.285
Expression system: Escherichia coli BL21(DE3)

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Citations

10 citation in other articles

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Uga et al. (2006)
9 more

1 mention without citation

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Ardiani et al. (2012)