› 2a3r

X-ray diffraction
2.6Å resolution

Crystal Structure of Human Sulfotransferase SULT1A3 in Complex with Dopamine and 3-Phosphoadenosine 5-Phosphate

Released:
DOI: 10.2210/pdb2a3r/pdb
PDB entry 2a3r coloured by chain, front view.
PDB entry 2a3r coloured by chain, side view.
PDB entry 2a3r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human sulfotransferase SULT1A3 in complex with dopamine and 3'-phosphoadenosine 5'-phosphate.
Lu JH, Li HT, Liu MC, Zhang JP, Li M, An XM, Chang WR
Biochem Biophys Res Commun 335 417-23 (2005)
PMID: 16083857

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143642 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfotransferase 1A3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 295 amino acids
Theoretical weight: 34.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0DMM9 (Residues: 1-295; Coverage: 100%)
Gene names: STM, SULT1A3
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand A3P 2 x A3P
Ligand LDP 2 x LDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL12B2
Spacegroup: P21212
Unit cell:
a: 79.263Å b: 123.61Å c: 73.116Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.206 0.243
Expression system: Escherichia coli BL21

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Citations

3 review citations

Substrate inhibition kinetics in drug metabolism reactions.
Wu B. (2011)
2 more

21 mentions without citation

The structural biology of oestrogen metabolism.
Thomas et al. (2013)
20 more